LC3B Polyclonal Antibody

Elabscience
Product Code: E-AB-68372
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-68372-60uL60uL£210.00
Quantity:
E-AB-68372-120uL120uL£291.00
Quantity:
E-AB-68372-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various lysates using LC3B Polyclonal Antibody at 1:2000 dilution.NIH/3T3 and C6 were treated by Chloroquine (50 uM) at 37°C for 20 hours.
2 / 2
Immunohistochemistry of paraffin-embedded Human breast cancer using LC3B Polyclonal Antibody at dilution of 1:250 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of extracts of various lysates using LC3B Polyclonal Antibody at 1:2000 dilution.NIH/3T3 and C6 were treated by Chloroquine (50 uM) at 37°C for 20 hours.
Immunohistochemistry of paraffin-embedded Human breast cancer using LC3B Polyclonal Antibody at dilution of 1:250 (20x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
LC3B
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
14kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human LC3B
ObservedMW:
14kDa/16kDa
Purification method:
Affinity purification
Target Synonym:
LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
UNIProt ID:
Q9GZQ8