CRYAA Polyclonal Antibody

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Elabscience
Product Code: E-AB-68378
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-68378-60uL60uL£210.00
Quantity:
E-AB-68378-120uL120uL£291.00
Quantity:
E-AB-68378-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of HepG2 using CRYAA Polyclonal Antibody at 1:5000 dilution.
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Immunofluorescence analysis of Rat Eye using CRYAA Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of HepG2 using CRYAA Polyclonal Antibody at 1:5000 dilution.
Immunofluorescence analysis of Rat Eye using CRYAA Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
CRYAA
Background:
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
19kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human CRYAA
ObservedMW:
19KDa
Purification method:
Affinity purification
Target Synonym:
CRYAA;CRYA1;CTRCT9;HSPB4
UNIProt ID:
P02489