53BP1 Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-68380
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-68380-60uL60uL£210.00
Quantity:
E-AB-68380-120uL120uL£291.00
Quantity:
E-AB-68380-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of HeLa cells using 53BP1 Polyclonal Antibody at 1:1000 dilution.
2 / 2
Immunohistochemistry of paraffin-embedded human brain using 53BP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
❮❯

Western blot analysis of extracts of HeLa cells using 53BP1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human brain using 53BP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
53BP1
Background:
Double-strand break (DSB repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs in response to DNA damage by promoting non-homologous end joining (NHEJ-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub and histone H4 dimethylated at 'Lys-20' (H4K20me2, two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity. In contrast, it is not required for classic NHEJ and V(DJ recombination (By similarity. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
213kDa/214kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human 53BP1
ObservedMW:
450KDa
Purification method:
Affinity purification
Target Synonym:
TP53BP1;53BP1;TDRD30;TP53;p202;p53BP1
UNIProt ID:
Q12888