CRB1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-90235
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-90235-60uL60uL£210.00
Quantity:
E-AB-90235-120uL120uL£291.00
Quantity:
E-AB-90235-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells using CRB1 Polyclonal Antibody at 1:1000 dilution.
2 / 2
Immunohistochemistry of paraffin-embedded mouse retina using CRB1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of HeLa cells using CRB1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse retina using CRB1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
CRB1
Background:
This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
95kDa/104kDa/142kDa/151kDa/154kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human CRB1
ObservedMW:
150kDa
Purification method:
Affinity purification
Target Synonym:
CRB1;LCA8;RP12
UNIProt ID:
P82279