NEK2 Polyclonal Antibody

Elabscience
Product Code: E-AB-90632
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-90632-60uL60uL£210.00
Quantity:
E-AB-90632-120uL120uL£291.00
Quantity:
E-AB-90632-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 2
Western blot analysis of extracts of HeLa cells using NEK2 Polyclonal Antibody at 1:1000 dilution.
2 / 2
Immunohistochemistry of paraffin-embedded rat ovary using NEK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of extracts of HeLa cells using NEK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using NEK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
NEK2
Background:
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
37kDa/44kDa/50kDa/51kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human NEK2
ObservedMW:
52kDa
Purification method:
Affinity purification
Target Synonym:
NEK2;HsPK21;NEK2A;NLK1;PPP1R111;RP67
UNIProt ID:
P51955