SP1 Polyclonal Antibody

Elabscience
Product Code: E-AB-90919
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-90919-60uL60uL£210.00
Quantity:
E-AB-90919-120uL120uL£291.00
Quantity:
E-AB-90919-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 2
Immunohistochemistry of paraffin-embedded rat liver using SP1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
2 / 2
Immunofluorescence analysis of NIH/3T3 cells using SP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded rat liver using SP1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using SP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
SP1
Background:
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
75kDa/79kDa/80kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:100-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human SP1
ObservedMW:
90KDa
Purification method:
Affinity purification
Target Synonym:
SP1
UNIProt ID:
P08047