Raptor Polyclonal Antibody

Elabscience
Product Code: E-AB-91499
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-91499-60uL60uL£210.00
Quantity:
E-AB-91499-120uL120uL£291.00
Quantity:
E-AB-91499-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Immunohistochemistry of paraffin-embedded mouse brain using Raptor Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
2 / 3
Immunofluorescence analysis of NIH/3T3 cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of PC-12 cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded mouse brain using Raptor Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Raptor
Background:
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
43kDa/131kDa/149kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human RPTOR
ObservedMW:
140-150kDa
Purification method:
Affinity purification
Target Synonym:
KOG1;Mip1;RPTOR
UNIProt ID:
Q8N122