Gelsolin Polyclonal Antibody

Elabscience
Product Code: E-AB-91640
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-91640-60uL60uL£210.00
Quantity:
E-AB-91640-120uL120uL£291.00
Quantity:
E-AB-91640-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines using Gelsolin Polyclonal Antibody at 1:1000 dilution.
2 / 7
Immunohistochemistry of paraffin-embedded human colon carcinoma using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 7
Immunohistochemistry of paraffin-embedded human liver cancer using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 7
Immunohistochemistry of paraffin-embedded mouse spleen using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 7
Immunohistochemistry of paraffin-embedded rat lung using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 7
Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of U2OS cells using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines using Gelsolin Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spleen using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Gelsolin
Background:
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
80kDa/81kDa/85kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Gelsolin
ObservedMW:
86KDa
Purification method:
Affinity purification
Target Synonym:
GSN;ADF;AGEL;gelsolin
UNIProt ID:
P06396