ITCH Polyclonal Antibody

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Elabscience
Product Code: E-AB-91779
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-91779-60uL60uL£210.00
Quantity:
E-AB-91779-120uL120uL£291.00
Quantity:
E-AB-91779-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines using ITCH Polyclonal Antibody at dilution.
2 / 3
Immunohistochemistry of paraffin-embedded human lung cancer using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 3
Immunohistochemistry of paraffin-embedded human placenta using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using ITCH Polyclonal Antibody at dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
ITCH
Background:
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
103kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100
Immunogen:
Recombinant fusion protein of human ITCH
ObservedMW:
100kDa
Purification method:
Affinity purification
Target Synonym:
ITCH;ADMFD;AIF4;AIP4;NAPP1
UNIProt ID:
Q96J02