TPX2 Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-92021
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-92021-60uL60uL£210.00
Quantity:
E-AB-92021-120uL120uL£291.00
Quantity:
E-AB-92021-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Immunohistochemistry of paraffin-embedded human colon carcinoma using TPX2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
2 / 3
Immunofluorescence analysis of C6 cells using TPX2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of U2OS cells using TPX2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Immunohistochemistry of paraffin-embedded human colon carcinoma using TPX2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using TPX2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using TPX2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
TPX2
Background:
Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
85kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human TPX2
ObservedMW:
105KDa
Purification method:
Affinity purification
Target Synonym:
DIL2;p100;DIL-2;HCTP4;FLS353;HCA519;REPP86;C20orf1;C20orf2;GD:C20orf1;TPX2
UNIProt ID:
Q9ULW0