HSP70 Polyclonal Antibody

Elabscience
Product Code: E-AB-92322
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-92322-60uL60uL£210.00
Quantity:
E-AB-92322-120uL120uL£291.00
Quantity:
E-AB-92322-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using HSP70  Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines using HSP70  Polyclonal Antibody at 1:1000 dilution.
3 / 7
Immunohistochemistry of paraffin-embedded human colon carcinoma using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 7
Immunohistochemistry of paraffin-embedded human esophageal cancer using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 7
Immunofluorescence analysis of A-549 cells using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
6 / 7
Immunofluorescence analysis of NIH/3T3 cells using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of PC-12 cells using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines using HSP70  Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using HSP70  Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-549 cells using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using HSP70 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
HSP70
Background:
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
70kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human HSP70
ObservedMW:
70KDa
Purification method:
Affinity purification
Target Synonym:
HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1
UNIProt ID:
P0DMV8