MAP2K4 Polyclonal Antibody

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Elabscience
Product Code: E-AB-92400
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-92400-60uL60uL£210.00
Quantity:
E-AB-92400-120uL120uL£291.00
Quantity:
E-AB-92400-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of A-549 using MAP2K4 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of various lysates using MAP2K4 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded Mouse liver using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
4 / 7
Immunohistochemistry of paraffin-embedded Rat ovary using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
5 / 7
Immunohistochemistry of paraffin-embedded Mouse kidney using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
6 / 7
Immunohistochemistry of paraffin-embedded Rat lung using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval wit
7 / 7
Immunofluorescence analysis of L929 cells using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of A-549 using MAP2K4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of various lysates using MAP2K4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse liver using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat ovary using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse kidney using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat lung using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval wit
Immunofluorescence analysis of L929 cells using MAP2K4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
MAP2K4
Background:
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
44kDa/45kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human MAP2K4
ObservedMW:
44KDa
Purification method:
Affinity purification
Target Synonym:
MAP2K4; JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1; mitogen-activated protein kinase kinase 4
UNIProt ID:
P45985