(KO Validated) hnRNP E1/PCBP1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-92424
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-92424-60uL60uL£224.00
Quantity:
E-AB-92424-120uL120uL£317.00
Quantity:
E-AB-92424-200uL200uL£465.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts from wild type(WT) and hnRNP E1/PCBP1 knockout (KO) HeLa cells using hnRNP E1/PCBP1 Polyclonal Antibody at 1:1000 dilution.
2 / 3
Immunohistochemistry of paraffin-embedded mouse testis using hnRNP E1/PCBP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 3
Immunohistochemistry of paraffin-embedded rat brain using hnRNP E1/PCBP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of extracts from wild type(WT) and hnRNP E1/PCBP1 knockout (KO) HeLa cells using hnRNP E1/PCBP1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse testis using hnRNP E1/PCBP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using hnRNP E1/PCBP1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
hnRNP E1/PCBP1
Background:
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
37kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human hnRNP E1/PCBP1
ObservedMW:
40KDa
Purification method:
Affinity purification
Target Synonym:
PCBP1; HEL-S-85; HNRPE1; HNRPX; hnRNP-E1; hnRNP-X; poly(rC)-binding protein 1
UNIProt ID:
Q15365