(KO Validated) Smad1 Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-92425
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-92425-60uL60uL£224.00
Quantity:
E-AB-92425-120uL120uL£317.00
Quantity:
E-AB-92425-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Western blot analysis of extracts from wild type(WT) and Smad1 knockout (KO) HeLa cells using Smad1 Polyclonal Antibody at 1:1000 dilution.
2 / 3
Immunohistochemistry of paraffin-embedded mouse lung using Smad1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 3
Immunohistochemistry of paraffin-embedded rat kidney using Smad1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
❮❯

Western blot analysis of extracts from wild type(WT) and Smad1 knockout (KO) HeLa cells using Smad1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse lung using Smad1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using Smad1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
Smad1
Background:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
15kDa/52kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Smad1
ObservedMW:
60KDa
Purification method:
Affinity purification
Target Synonym:
SMAD1; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; SMAD family member 1
UNIProt ID:
Q15797