Lamin A/C Polyclonal Antibody

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Elabscience
Product Code: E-AB-93040
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93040-120uL120uL£291.00
Quantity:
E-AB-93040-200uL200uL£431.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 4
Immunohistochemistry of paraffin-embedded human breast cancer using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
2 / 4
Immunofluorescence analysis of A-549 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of PC-12 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human breast cancer using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-549 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Lamin A/C
Background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
62-74kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Lamin A/C
ObservedMW:
68KDa,72KDa
Purification method:
Affinity purification
Target Synonym:
LMNA;CDCD1;CDDC;CMD1A;CMT2B1;EMD2;FPL;FPLD;FPLD2;HGPS;IDC;LDP1;LFP;LGMD1B;LMN1;LMNC;LMNL1;MADA;PRO1;lamin;Lamin A/C
UNIProt ID:
P02545