Alkaline Phosphatase (ALPL) Polyclonal Antibody

Elabscience
Product Code: E-AB-93077
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93077-60uL60uL£210.00
Quantity:
E-AB-93077-120uL120uL£291.00
Quantity:
E-AB-93077-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells using Alkaline Phosphatase Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded mouse kidney using Alkaline Phosphatase Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of extracts of HeLa cells using Alkaline Phosphatase Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse kidney using Alkaline Phosphatase Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
Alkaline Phosphatase (ALPL)
Background:
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
48kDa/51kDa/57kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human Alkaline Phosphatase
ObservedMW:
80KDa
Purification method:
Affinity purification
Target Synonym:
ALPL;AP-TNAP;APTNAP;HOPS;TNAP
UNIProt ID:
P05186