DNMT1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93132
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93132-60uL60uL£210.00
Quantity:
E-AB-93132-120uL120uL£291.00
Quantity:
E-AB-93132-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines using DNMT1 Polyclonal Antibody at 1:1000 dilution.
2 / 4
Immunohistochemistry of paraffin-embedded rat spleen using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 4
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
4 / 4
Immunohistochemistry of paraffin-embedded human lung cancer using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using DNMT1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat spleen using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
DNMT1
Background:
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
144kDa/183kDa/184kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IP 1:50-1:200
Immunogen:
Recombinant fusion protein of human DNMT1
ObservedMW:
200KDa
Purification method:
Affinity purification
Target Synonym:
ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
UNIProt ID:
P26358