CHRM1 Polyclonal Antibody

Elabscience
Product Code: E-AB-93142
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93142-60uL60uL£210.00
Quantity:
E-AB-93142-120uL120uL£291.00
Quantity:
E-AB-93142-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Immunohistochemistry of paraffin-embedded mouse brain using CHRM1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
2 / 3
Immunofluorescence analysis of Neuro-2a cells using CHRM1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of SH-SY5Y cells using CHRM1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded mouse brain using CHRM1 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Neuro-2a cells using CHRM1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of SH-SY5Y cells using CHRM1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
CHRM1
Background:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
50kDa/51kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:500-1:2000,IF 1:50-1:200
Immunogen:
A synthetic peptide of human CHRM1
ObservedMW:
51KDa
Purification method:
Affinity purification
Target Synonym:
CHRM1;HM1;M1;M1R
UNIProt ID:
P11229