Cyclin B1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93144
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93144-60uL60uL£210.00
Quantity:
E-AB-93144-120uL120uL£291.00
Quantity:
E-AB-93144-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 8
Western blot analysis of extracts of various cell lines using Cyclin B1 Polyclonal Antibody at 1:500 dilution.
2 / 8
Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 8
Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
4 / 8
Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
5 / 8
Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
6 / 8
Immunofluorescence analysis of HeLa cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
7 / 8
Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
8 / 8
Immunofluorescence analysis of PC-12 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using Cyclin B1 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Cyclin B1
Background:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
44kDa/48kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human Cyclin B1
ObservedMW:
55KDa
Purification method:
Affinity purification
Target Synonym:
CCNB;CCNB1;Cyclin B1;cyclin B1
UNIProt ID:
P14635