p70 S6 Kinase 1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93156
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93156-60uL60uL£210.00
Quantity:
E-AB-93156-120uL120uL£291.00
Quantity:
E-AB-93156-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 6
Western blot analysis of extracts of various cell lines using p70 S6 Kinase 1 1 Polyclonal Antibody at 1:1000 dilution.
2 / 6
Immunohistochemistry of paraffin-embedded rat brain using p70 S6 Kinase 1 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 6
Immunohistochemistry of paraffin-embedded human breast cancer using p70 S6 Kinase 1 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol
4 / 6
Immunohistochemistry of paraffin-embedded mouse lung using p70 S6 Kinase 1 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 6
Immunofluorescence analysis of C6 cells using p70 S6 Kinase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of NIH/3T3 cells using p70 S6 Kinase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using p70 S6 Kinase 1 1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using p70 S6 Kinase 1 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using p70 S6 Kinase 1 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol
Immunohistochemistry of paraffin-embedded mouse lung using p70 S6 Kinase 1 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using p70 S6 Kinase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using p70 S6 Kinase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
p70 S6 Kinase 1
Background:
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
51kDa/52kDa/56kDa/59kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human P70 S6K
ObservedMW:
70KDa
Purification method:
Affinity purification
Target Synonym:
PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA
UNIProt ID:
P23443