(KO Validated) CDK4 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93172
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93172-60uL60uL£224.00
Quantity:
E-AB-93172-120uL120uL£317.00
Quantity:
E-AB-93172-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 5
Immunohistochemistry of paraffin-embedded mouse lung using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 5
Immunohistochemistry of paraffin-embedded rat testis using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 5
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate usin
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Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate usin

Documents

Further Information

Abbreviation:
CDK4
Background:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
20kDa/33kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IP 1:50-1:200
Immunogen:
A synthetic peptide of human CDK4
ObservedMW:
30KDa
Purification method:
Affinity purification
Target Synonym:
CDK4;CMM3;PSK-J3
UNIProt ID:
P11802