(KO Validated) CDK2 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93179
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93179-60uL60uL£224.00
Quantity:
E-AB-93179-120uL120uL£317.00
Quantity:
E-AB-93179-200uL200uL£465.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using CDK2 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells using CDK2 Polyclonal Antibody at 1:1000 dilution.
3 / 7
Western blot analysis of extracts of HeLa cells using CDK2 Polyclonal Antibody at 1:1000 dilution.
4 / 7
Immunohistochemistry of paraffin-embedded human colon carcinoma using CDK2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 7
Immunofluorescence analysis of HeLa cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
6 / 7
Immunofluorescence analysis of PC-12 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of U2OS cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using CDK2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells using CDK2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of HeLa cells using CDK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CDK2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
CDK2
Background:
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
30kDa/33kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human CDK2
ObservedMW:
33kDa
Purification method:
Affinity purification
Target Synonym:
CDK2;CDKN2;p33(CDK2)
UNIProt ID:
P24941