IRE1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93217
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93217-60uL60uL£210.00
Quantity:
E-AB-93217-120uL120uL£291.00
Quantity:
E-AB-93217-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of 293T cells using IRE1 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines using IRE1 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human breast cancer using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 8
Immunohistochemistry of paraffin-embedded human colon carcinoma using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 8
Immunohistochemistry of paraffin-embedded mouse kidney using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 8
Immunohistochemistry of paraffin-embedded rat kidney using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval
7 / 8
Immunohistochemistry of paraffin-embedded rat pancreas using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retriev
8 / 8
Immunofluorescence analysis of U2OS cells using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 293T cells using IRE1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using IRE1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon carcinoma using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval
Immunohistochemistry of paraffin-embedded rat pancreas using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retriev
Immunofluorescence analysis of U2OS cells using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
IRE1
Background:
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
6kDa/109kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic phosphorylated of human IRE1
ObservedMW:
130KDa
Purification method:
Affinity purification
Target Synonym:
ERN1;IRE1;IRE1P;IRE1a;hIRE1p
UNIProt ID:
O75460