JNK1/2/3 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93242
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93242-60uL60uL£210.00
Quantity:
E-AB-93242-120uL120uL£291.00
Quantity:
E-AB-93242-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Immunohistochemistry of paraffin-embedded human lung cancer using JNK1/2/3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
2 / 3
Immunofluorescence analysis of U2OS cells using JNK1/2/3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using JNK1/2/3 Polyclonal Antibody at a dilution of 1:1000.
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Immunohistochemistry of paraffin-embedded human lung cancer using JNK1/2/3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using JNK1/2/3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using JNK1/2/3 Polyclonal Antibody at a dilution of 1:1000.

Documents

Further Information

Abbreviation:
JNK1/2/3
Background:
JNK belongs to MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1,JNK2,and JNK3
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
35kDa/44kDa/48kDa/27kDa/52kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:100
Immunogen:
Recombinant fusion protein of human JNK1/2/3
ObservedMW:
46kDa/54kDa
Purification method:
Affinity purification
UNIProt ID:
P45983/P45984/P53779