BRD4 Polyclonal Antibody

Elabscience
Product Code: E-AB-93245
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93245-60uL60uL£210.00
Quantity:
E-AB-93245-120uL120uL£291.00
Quantity:
E-AB-93245-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Western blot analysis of extracts of HeLa cells using BRD4 Polyclonal Antibody at 1:1000 dilution.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using BRD4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 3
Immunohistochemistry of paraffin-embedded rat testis using BRD4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of extracts of HeLa cells using BRD4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human stomach using BRD4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using BRD4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Documents

Further Information

Abbreviation:
BRD4
Background:
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
80kDa/88kDa/152kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human BRD4
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
BRD4;CAP;HUNK1;HUNKI;MCAP
UNIProt ID:
O60885