Rad21 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93246
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93246-60uL60uL£210.00
Quantity:
E-AB-93246-120uL120uL£291.00
Quantity:
E-AB-93246-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using Rad21 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of C6 cells using Rad21 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 8
Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 8
Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 8
Immunofluorescence analysis of U2OS cells using Rad21 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
7 / 8
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate u
8 / 8
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate u
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Western blot analysis of extracts of various cell lines using Rad21 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of C6 cells using Rad21 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Rad21 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate u
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate u

Documents

Further Information

Abbreviation:
Rad21
Background:
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
71kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:200
Immunogen:
Recombinant fusion protein of human Rad21
ObservedMW:
130KDa
Purification method:
Affinity purification
Target Synonym:
CDLS4; HR21; HRAD21; MCD1; NXP1; SCC1; hHR21
UNIProt ID:
O60216