CASP1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93260
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93260-60uL60uL£210.00
Quantity:
E-AB-93260-120uL120uL£291.00
Quantity:
E-AB-93260-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Western blot analysis of extracts of Mouse testis using CASP1 Polyclonal Antibody at 1:1000 dilution.
2 / 3
Immunofluorescence analysis of C6 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Mouse testis using CASP1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CASP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
CASP1
Background:
Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B and interleukin 18 (IL18 as well as the pyroptosis inducer Gasdermin-D (GSDMD, into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (By similarity.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
45kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IF 1:50-1:200
Immunogen:
A synthetic peptide of mouse CASP1
ObservedMW:
48KDa
Purification method:
Affinity purification
Target Synonym:
IC; ICE; Il1; Casp; Il1bc
UNIProt ID:
P29452