(KO Validated) Caspase-3 p17 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93323
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93323-60uL60uL£224.00
Quantity:
E-AB-93323-120uL120uL£317.00
Quantity:
E-AB-93323-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of Mouse lung using Caspase-3 p17 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of Jurkat cells using Caspase-3 p17 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by staurosporine(1 uM) for 3 hour.
3 / 7
Western blot analysis of extracts from wild type(WT) and Caspase-3 p17 knockout (KO) 293T cells using Caspase-3 p17 Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded mouse lung using Caspase-3 p17 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 7
Immunofluorescence analysis of L929 cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC-12 cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Mouse lung using Caspase-3 p17 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Jurkat cells using Caspase-3 p17 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by staurosporine(1 uM) for 3 hour.
Western blot analysis of extracts from wild type(WT) and Caspase-3 p17 knockout (KO) 293T cells using Caspase-3 p17 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse lung using Caspase-3 p17 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Caspase-3 p17
Background:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
31kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Caspase-3
ObservedMW:
32KDa/17KDa
Purification method:
Affinity purification
Target Synonym:
CASP3; CPP32; CPP32B; SCA-1; caspase-3
UNIProt ID:
P42574