(KO Validated) Beclin 1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93325
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93325-60uL60uL£224.00
Quantity:
E-AB-93325-120uL120uL£317.00
Quantity:
E-AB-93325-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines using Beclin 1 Polyclonal Antibody at 1:1000 dilution.
2 / 5
Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 5
Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
4 / 5
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using Beclin 1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using [KO Validated] Beclin 1 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Beclin 1
Background:
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
51kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Beclin 1
ObservedMW:
60KDa
Purification method:
Affinity purification
Target Synonym:
BECN1; ATG6; VPS30; beclin1; beclin-1
UNIProt ID:
Q14457