(KD Validated) PROM1 Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-93326
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93326-60uL60uL£224.00
Quantity:
E-AB-93326-120uL120uL£317.00
Quantity:
E-AB-93326-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 8
Western blot analysis of extracts of HT-29 cells using PROM1 Polyclonal Antibody at 1:400 dilution.
2 / 8
Western blot analysis of extracts from wild type(WT) and PROM1 knockdown (KD) HCT116 cells using PROM1 Polyclonal Antibody at 1:400 dilution.
3 / 8
4 / 8
Immunohistochemistry of paraffin-embedded rat kidney using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 8
Immunohistochemistry of paraffin-embedded rat lung using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 8
Immunofluorescence analysis of C6 cells using CD133 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
7 / 8
Immunofluorescence analysis of HT-29 cells using PROM1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
8 / 8
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 Polyclonal Antibody. Western blot was performed from the immunoprecipitate us
❮❯

Western blot analysis of extracts of HT-29 cells using PROM1 Polyclonal Antibody at 1:400 dilution.
Western blot analysis of extracts from wild type(WT) and PROM1 knockdown (KD) HCT116 cells using PROM1 Polyclonal Antibody at 1:400 dilution.
Immunohistochemistry of paraffin-embedded rat kidney using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using CD133 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using CD133 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HT-29 cells using PROM1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 Polyclonal Antibody. Western blot was performed from the immunoprecipitate us

Documents

Further Information

Abbreviation:
PROM1
Background:
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
92kDa/93kDa/94kDa/96kDa/97kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200,IP 50-1:200
Immunogen:
Recombinant fusion protein of human PROM1
ObservedMW:
120KDa
Purification method:
Affinity purification
Target Synonym:
PROM1; AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4; prominin-1
UNIProt ID:
O43490