(KO Validated) SQSTM1/p62 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93327
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93327-60uL60uL£224.00
Quantity:
E-AB-93327-120uL120uL£317.00
Quantity:
E-AB-93327-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using SQSTM1/p62 Polyclonal Antibody at 1:4000 dilution.
2 / 7
Western blot analysis of extracts from wild type(WT) and SQSTM1/p62 knockout (KO) 293T cells using SQSTM1/p62 Polyclonal Antibody at 1:4000 dilution.
3 / 7
Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 7
Immunohistochemistry of paraffin-embedded Human extranodal NK-T cell lymphoma using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
5 / 7
Immunohistochemistry of paraffin-embedded Mouse kidney using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 7
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of C6 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using SQSTM1/p62 Polyclonal Antibody at 1:4000 dilution.
Western blot analysis of extracts from wild type(WT) and SQSTM1/p62 knockout (KO) 293T cells using SQSTM1/p62 Polyclonal Antibody at 1:4000 dilution.
Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human extranodal NK-T cell lymphoma using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining
Immunohistochemistry of paraffin-embedded Mouse kidney using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
SQSTM1/p62
Background:
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
38kDa/47kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human SQSTM1/p62
ObservedMW:
62KDa
Purification method:
Affinity purification
Target Synonym:
SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B; sequestosome-1
UNIProt ID:
Q13501