(KD Validated) TOM20 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93329
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93329-60uL60uL£224.00
Quantity:
E-AB-93329-120uL120uL£317.00
Quantity:
E-AB-93329-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using TOM20 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines using [KD Validated] TOM20 Polyclonal Antibody at 1:1000 dilution.
3 / 8
Western blot analysis of extracts from wild type(WT) and TOM20 knockdown (KD) 293T cells using (KD Validated)TOM20 Polyclonal Antibody at 1:1000 dilution.
4 / 8
Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 8
Immunohistochemistry of paraffin-embedded mouse spleen using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 8
Immunohistochemistry of paraffin-embedded rat brain using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval
7 / 8
Immunohistochemistry of paraffin-embedded rat kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval
8 / 8
Immunofluorescence analysis of C6 cells using TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using TOM20 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using [KD Validated] TOM20 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from wild type(WT) and TOM20 knockdown (KD) 293T cells using (KD Validated)TOM20 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spleen using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval
Immunohistochemistry of paraffin-embedded rat kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval
Immunofluorescence analysis of C6 cells using TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
TOM20
Background:
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
16kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human TOM20
ObservedMW:
16KDa
Purification method:
Affinity purification
Target Synonym:
TOMM20; MAS20; MOM19; TOM20; translocase of outer mitochondrial membrane 20
UNIProt ID:
Q15388