CDK1 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93330
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93330-60uL60uL£210.00
Quantity:
E-AB-93330-120uL120uL£291.00
Quantity:
E-AB-93330-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

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Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of Jurkat cells using CDK1 Polyclonal Antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.
4 / 6
Immunofluorescence analysis of C6 cells using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 6
Immunoprecipitation analysis of 600ug extracts of Jurkat cells using 3ug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate us
6 / 6
Immunoprecipitation analysis of 600ug extracts of C6 cells using 3ug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using
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Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Jurkat cells using CDK1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of Jurkat cells using 3ug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate us
Immunoprecipitation analysis of 600ug extracts of C6 cells using 3ug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using

Documents

Further Information

Abbreviation:
CDK1
Background:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
27kDa/34kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IF 1:50-1:200,IP 50-1:200
Immunogen:
A synthetic peptide of human CDK1
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
CDK1; CDC2; CDC28A; P34CDC2; cyclin-dependent kinase 1
UNIProt ID:
P06493