AMPKα1 Polyclonal Antibody

Elabscience
Product Code: E-AB-93332
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93332-60uL60uL£210.00
Quantity:
E-AB-93332-120uL120uL£291.00
Quantity:
E-AB-93332-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Immunofluorescence (IF)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 3
Immunofluorescence analysis of HeLa cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
2 / 3
Immunofluorescence analysis of NIH/3T3 cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of PC-12 cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue:DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
AMPKα1
Background:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
64kDa/65kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
IF 1:50-1:20
Immunogen:
A synthetic peptide of human AMPKα1
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
UNIProt ID:
Q13131