SHMT2 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93343
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93343-60uL60uL£210.00
Quantity:
E-AB-93343-120uL120uL£291.00
Quantity:
E-AB-93343-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 5
Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
2 / 5
Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
3 / 5
Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
4 / 5
Immunofluorescence analysis of L929 cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SHMT2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate usi
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Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SHMT2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate usi

Documents

Further Information

Abbreviation:
SHMT2
Background:
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
53kDa/54kDa/55kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
IHC 1:50-1:100,IF1:50-1:200,IP 1:50-1:200
Immunogen:
Recombinant fusion protein of human SHMT2
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
SHMT2; GLYA; HEL-S-51e; SHMT; serine hydroxymethyltransferase 2
UNIProt ID:
P34897