Cleaved Caspase-9 P37 Polyclonal Antibody

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Elabscience
Product Code: E-AB-93363
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93363-60uL60uL£210.00
Quantity:
E-AB-93363-120uL120uL£291.00
Quantity:
E-AB-93363-200uL200uL£431.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 6
Western blot analysis of extracts of Jurkat using Cleaved Caspase-9 P37 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours.
2 / 6
Immunohistochemistry of paraffin-embedded human liver cancer using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 6
Immunohistochemistry of paraffin-embedded mouse kidney using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 6
Immunohistochemistry of paraffin-embedded rat kidney using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 6
Immunofluorescence analysis of HepG2 cells using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue:DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of PC-12 cells using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue:DAPI for nuclear staining.
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Western blot analysis of extracts of Jurkat using Cleaved Caspase-9 P37 Polyclonal Antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours.
Immunohistochemistry of paraffin-embedded human liver cancer using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue:DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Cleaved Caspase-9 P37 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue:DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
Cleaved Caspase-9 P37
Background:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
17kDa/30kDa/36kDa/46kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Cleaved Caspase-9 P37
ObservedMW:
35kDa/37kDa
Purification method:
Affinity purification
Target Synonym:
CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
UNIProt ID:
P55211