(KD Validated) eIF4E Polyclonal Antibody

Contact us
Elabscience
Product Code: E-AB-93365
Product Group: Primary Antibodies
Supplier: Elabscience
CodeSizePrice
E-AB-93365-60uL60uL£224.00
Quantity:
E-AB-93365-120uL120uL£317.00
Quantity:
E-AB-93365-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Ice packs
Storage:
Store at -20°C. Avoid freeze/thaw cycles.

Images

1 / 8
Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using eIF4E Polyclonal Antibody at 1:500 dilution
2 / 8
Immunohistochemistry of paraffin-embedded Rat testis using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 8
Immunohistochemistry of paraffin-embedded Mouse liver using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 8
Immunohistochemistry of paraffin-embedded Human liver using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 8
Immunofluorescence analysis of HeLa cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
6 / 8
Immunofluorescence analysis of HepG2 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
7 / 8
Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining
8 / 8
Immunofluorescence analysis of PC-12 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using eIF4E Polyclonal Antibody at 1:500 dilution
Immunohistochemistry of paraffin-embedded Rat testis using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse liver using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human liver using EIF4E Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining
Immunofluorescence analysis of PC-12 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Abbreviation:
eIF4E
Background:
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
25kDa/27kDa/28kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IF 1:50-1:200,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human eIF4E
ObservedMW:
29KDa
Purification method:
Affinity purification
Target Synonym:
AUTS19;CBP;EIF4E1;EIF4EL1;EIF4F;eIF-4E;EIF4E
UNIProt ID:
P06730