Pyruvate Dehydrogenase (PDH) Activity Assay Kit
| Code | Size | Price |
|---|
| E-BC-K650-M-96T | 96T | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
15 min
Detection Instrument:
Microplate reader
Detection Range:
0.28-76.23 U/L
Detection Type:
Colorimetric method
Inter cv:
10
Intra cv:
2
Result Type:
Enzyme Activity
Sensitivity:
0.28 U /L
Target Synonym:
PDH
Test Principle:
Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
Tested Sample Types:
serum (plasma), urine, animal tissue,cell