Plant Root Activity Assay Kit
| Code | Size | Price |
|---|
| E-BC-K855-S-100Assays | 100 Assays | £224.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
3.5h
Detection Instrument:
Spectrophotometer
Detection Range:
0-0.67 U/g wet weight
Detection Type:
Colorimetric method
Inter cv:
3
Intra cv:
1
Result Type:
Enzyme Activity
Sensitivity:
0 U/g wet weight
Test Principle:
The detection principle of the kit is to use 2, 3, 5-triphenyltetrazolium chloride (TTC) as the substrate, incubate for 1 to 3 hours, the dehydrogenase in the root can reduce TTC and generate 1,3,5-tiphenylformazan (TTF) which is insoluble in water, then TTF will be extracted from the root with organic solvent (ethyl acetate or acetone, etc.) By detecting the absorbance at 485nm, the amount of TTC reduction can be calculated, which represents the dehydrogenase activity and is used as an index of plant root activity. The kit is used for content determination of plant root activity or dehydrogenase activity.
Tested Sample Types:
plant tissue