Human PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit

PYGM

3.5h

This kit recognizes Human PYGM in samples. No significant cross-reactivity or interference between Human PYGM and analogues was observed

0.31-20 ng/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

0.19 ng/mL

Human

GPMM;Phosphorylase;Glycogen;McArdle Syndrome;Glycogen Storage Disease Type V;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PYGM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYGM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYGM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human PYGM. You can calculate the concentration of Human PYGM in the samples by comparing the OD of the samples to the standard curve.

Serum, plasma and other biological fluids

Sandwich

P11217

This ELISA kit applies to the in vitro quantitative determination of Human PYGM concentrations in Serum, plasma and other biological fluids.