Recombinant Human NEF3 protein (GST tag)

Elabscience
Product Code: PDEH100124
Product Group: Recombinant Proteins
Supplier: Elabscience
CodeSizePrice
PDEH100124-20ug20ug£113.00
Quantity:
PDEH100124-100ug100ug£140.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Human
Regulatory Status: RUO
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

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Further Information

Abbreviation:
NEF3
Accession:
P07197
Background:
Human NF-M (neurofilament-medium length) is a 145-150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2-358, which includes most of the rod region, shares ~98% aa sequence identity with corresponding region of mouse, rat and canine NF-M.
Calculated MW:
64.16 kDa
Expression Host:
E.coli
Formulation:
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-GST
ObservedMW:
60 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ser 2-Ser 358
Target Synonym:
Neurofilament medium polypeptide;NF-M;160 kDa neurofilament protein;Neurofilament 3;Neurofilament triplet M protein;NEFM;NEF3;NFM
UNIProt ID:
P07197