Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)
| Code | Size | Price |
|---|
| PDEO100002-20ug | 20ug | £113.00 |
Quantity:
| PDEO100002-100ug | 100ug | £140.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: E.coli
Regulatory Status: RUO
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
LacI
Accession:
P03023
Background:
Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Calculated MW:
39.4 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 um filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
40 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met1-Gln360
Target Synonym:
Lactose operon repressor;LacI
UNIProt ID:
P03023