Recombinant Human Myeloperoxidase/MPO Protein (His Tag)
| Code | Size | Price |
|---|
| PDMH100014-20ug | 20ug | £269.00 |
Quantity:
| PDMH100014-100ug | 100ug | £397.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
Myeloperoxidase;MPO
Accession:
P05164
Background:
Myeloperoxidase (MPO) is a heme-containing
enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that
catalyzes the hydrogen peroxide-dependent
conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational
processing of MPO involves
the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked
dimer composed of a
60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond
between the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of
the signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the
formation of 3-chlorotyrosine
and dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels
of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic
granules. Upon cellular activation, it is deposited into pathogen-containing
phagosomes.
Calculated MW:
80.3 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from a 0.2 um filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-10His
ObservedMW:
85-95 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Ala49-Ser745
Target Synonym:
Myeloperoxidase;MPO
UNIProt ID:
P05164