Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)(Active)
| Code | Size | Price |
|---|
| PKSH030396-50ug | 50ug | £652.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Further Information
Abbreviation:
c-MET;HGFR
Accession:
P08581-1
Activity:
1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.
2. Immobilized human HGFR (aa 956-1390) at 10 ug/ml (100 ul/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml.
Background:
Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
Calculated MW:
76.8 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
Baculovirus-Insect Cells
Formulation:
Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-His & GST
ObservedMW:
68 kDa
Purity:
> 90 % as determined by reducing SDS-PAGE.
Sequence:
Lys956-Ser1390
Target Synonym:
AUTS9;c-Met;DFNB97;HGFR;RCCP2
UNIProt ID:
P08581