Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag)
| Code | Size | Price |
|---|
| PKSH031276-50ug | 50ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
PRMT5;SKB1
Accession:
NP_006100.2
Background:
Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation; signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family; and exists as at least homodimers and homotetramers; or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development; as well as histone H3 'Arg-8'; which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally; it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.
Calculated MW:
75 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM EDTA, 0.5PMSF, 0.5mM, TCEP, 25% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His & N-FLAG
ObservedMW:
65 kDa
Purity:
> 85 % as determined by reducing SDS-PAGE.
Sequence:
Ala 2-Leu 637
Target Synonym:
HRMT1L5;IBP72;JBP1;SKB1;SKB1Hs