Recombinant Human MFG-E8/lactadherin/MFGE8 Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031387-100ug | 100ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Cell Culture
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
MFG-E8;lactadherin;MFGE8
Accession:
Q08431-1
Activity:
When 5 x 104 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 ug/mL, 100 uL/well), 45-85% cells will adhere after 1 hour at 37°C.
Background:
MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.
Calculated MW:
42 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
Baculovirus-Insect Cells
Formulation:
Lyophilized from sterile PBS, 0.2M Arg, 0.01% Tween-20, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
45 kDa
Purity:
> 80 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Cys 387
Target Synonym:
BA46;EDIL1;HMFG;hP47;HsT19888;MFG-E8;MFGM;OAcGD3S;SED1;SPAG10
UNIProt ID:
Q08431