Recombinant Human VEGFR3/FLT4 Protein (Fc Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031420-100ug | 100ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
VEGFR3;FLT4
Accession:
NP_002011.2
Activity:
1. Immobilized recombinant human VEGFR3 at 5 ug/ml (100 ul/well) can bind recombinant human VEGF-D at a linear range of 62. 5-2000 ng/ml.
2. Immobilized recombinant human VEGF-C at 10 ug/ml (100 ul/well) can bind recombinant human VEGFR3 at a linear range of 0.64-80 ng/ml.
3. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1. 4 nM.
Background:
Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.
Calculated MW:
111 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-hFc
ObservedMW:
160, 85, 75 kDa
Purity:
> 97 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ile 776
Target Synonym:
FLT-4;FLT-41;FLT41;LMPH1A;PCL;VEGF Receptor 3;VEGFR-3;VEGFR3