Recombinant Human PDGFRa/CD140a Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031528-100ug | 100ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
PDGFRa;CD140a
Accession:
NP_006197.1
Activity:
Measured by its ability to bind human PDGFC-Fc in functional ELISA.
Background:
PDGFRA; also known as CD140a; together with the structurally homolog protein PDGFRB (CD140b); are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell; and binds all three ligand isoforms PDGF-AA; PDGF-BB and PDGF-AB with high affinity; whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation; chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation; and in addition; particularly important for kidney development. Additionally; PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.
Calculated MW:
57.7 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
90-100 kDa
Purity:
> 97 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Glu 524
Target Synonym:
CD140A;PDGFR-2;PDGFR2;RHEPDGFRA;Platelet-derived growth factor receptor alpha;PDGFR-alpha;Alpha platelet-derived growth factor receptor;CD140 antigen-like family member A;Platelet-derived growth factor alpha receptor;Platelet-derived growth factor receptor 2