Recombinant Human TROP2/TACSTD2 Protein (His & Fc Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031614-100ug | 100ug | £441.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Applications:
- Cell Culture
- Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
TROP2;TACSTD2
Accession:
NP_002344.2
Activity:
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to coated plates (10ug/ml, 100ul/well), approximately 55%-70% will adhere specifically.
Background:
TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.
Calculated MW:
55.7 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His & Fc
ObservedMW:
65-75 kDa
Purity:
> 98 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Thr 274
Target Synonym:
EGP-1;EGP1;GA733-1;GA7331;GP50;M1S1;TACD2;TROP-2;TROP2