Recombinant Human JAM-A/F11R Protein (His Tag)(Active)
| Code | Size | Price |
|---|
| PKSH031771-100ug | 100ug | £664.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Applications:
- Cell Culture
- Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Further Information
Abbreviation:
JAM-A;F11R
Accession:
NP_058642.1
Activity:
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 104cells/well are added to JAM-A-Fc coated plates (2. 5ug/mL, 100 uL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37°C.
Background:
Junctional adhesion molecule-A (JAM-A); also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321); is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells; as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1; involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily; JAM-A protein involves in platelet adhesion; secretion and aggregation; and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition; it may be a potential therapeutic target for breast cancer.
Calculated MW:
25 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
28-32 kDa
Purity:
> 97 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ala 242
Target Synonym:
Junctional Adhesion Molecule A;JAM-A;Junctional Adhesion Molecule 1;JAM-1;Platelet F11 Receptor;Platelet Adhesion Molecule 1;PAM-1;CD321;F11R;JAM1;JCAM;JAMA;JCAM;KAT